Structure of O-glycosidically linked sugar units from plasma membranes of an ascites hepatoma, AH 66.

Structures of sialylated O-glycosidically and N-glycosidically linked oligosaccharides in a monoclonal immunoglobulin light chain.

The complete structures of the oligosaccharides in a human X-type immunoglobulin light chain have been determined. Four glycopeptides were isolated by chromatography on Bio-Gel P-6 columns following digestion of the glycoprotein with pronase. The 0-glycosidically linked chains were also isolated as reduced oligosaccharides by treating the glycoprotein with alkaline borohydride. Analysis of the ...

Submicroscopic structure of Yoshida ascites hepatoma.

The electron microscope study of u ascites hepatoma cells confirms their epithelial origin. Isolated cells may occur, but they are mostly gathered to form islets. Cell surfaces exhibit microvilli. Cells are interconnected by means of microvilli and of structures similar to the epithelial "terminal bars." The cells line spaces and canaliculi similar to bile canaliculi. The cytoplasm is poorly di...

Structure of O-glycosidically linked oligosaccharides from glycoproteins of Trypanosoma cruzi CL-Brener strain: evidence for the presence of O-linked sialyl-oligosaccharides.

Glycoproteins on the cell surface of Trypanosoma cruzi are known to play important roles in the interaction of the parasite with the host cells. We previously determined the structures of the O-glycan chains from the sialoglycoproteins (mucin-like molecules) of the G- and Y-strains and observed significant differences between them. We now report the structures of the sialylated and nonsialylate...

Metabolic-independent volume changes and Mg++ binding in mitochondria isolated from AH-130 Yoshida ascites hepatoma.

The mitochondria isolated in isotonic sucrose from AH-130 Yoshida ascites hepatoma appear spontaneously swollen (11, 12). This spontaneous swelling may be in part responsible for the inability of tumor mitochondria to undergo extensive volume changes in hypotonie conditions or in conditions that induce large-amplitude, metabolic-dependent, or metabolic-independent swelling in normal liver mitoc...

Lipoproteins isolated from plasma membranes of Morris hepatoma 5123.